Coding

Part:BBa_K2449014

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-09-28)


cex with a hlyA-tag, with a G-linker

This part consist of cenA with a hlyA-tag. The cenA gene encodes for the endoglucanse. The endoglucanase is together with the exoglucanase able to break down cellulose to cellubiose. The hlyA-tag makes the protein secreteable with the alpha-hemolysin transport system. Is is use in following brick BBa_K2449026 together with cenA (endoglucanse) BBa_K2449013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 624
    Illegal BamHI site found at 1573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None